||Virus and Cell Biology
||The First Affiliated Hospital
||Cell proliferation and differentiation; Antiviral research and drug development
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Ying-Jie Wang received his Ph.D. in Medical Biochemistry from Flinders University, Australia in 2001, followed by a three-year postdoctoral fellowship at the University of Texas Southwestern Medical Center in Dallas and a five-year tenure as a Senior Research Biochemist with Merck Research Laboratories, Merck & Co., Inc.. He is currently a professor at the School of Medicine, Zhejiang University as well as a Principal Investigator of the State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital of Zhejiang University School of Medicine. Dr. Wang is an author/inventor of over 40 research papers/meeting abstracts/patent applications, and a recipient of major awards including the First Prize of National Science and Technology Progress Award. He also led or participated in the pre-clinical development of multiple anti-HIV drug candidates. Currently, his research focuses on three main areas: 1) to decipher the post-translational modification barcodes of master pluripotency factors (e.g.,Oct4) in the contexts of stem cell self-renewal and differentiation; 2) to unravel the mechanisms by which master pluripotency factors (e.g.,Oct4) contribute to the generation of cancer stem cells and to develop corresponding therapeutic interventions targeting cancer stem cells; 3) to evaluate the roles of novel host factors in the life cycle of HIV-1 and HBV, and to develop specific small-molecule/peptide inhibitors or antibodies to combat these infectious pathogens.
1. Cheng J, Li W, Kang B, Zhou Y, Song J, Dan S, Yang Y, Zhang X, Li J, Yin S, Yao H, Zhu C, Yi W, Zhao Q, Xu X, Zheng M, Zheng S, Li L, Shen B, Wang YJ. (2015) Tryptophan derivatives regulate the transcription of Oct4 in stem-like cancer cells. Nature Communications, in press.
2. Zhao QW, Zhou YW, Li WX, Yang Y, Cheng J, Zhang XQ, Yin SY, Tong Y, He JQ, Kang B, Yao HP, Zheng M, Wang YJ. (2015) Akt-mediated phosphorylation of Oct4 is associated with the proliferation of stem-like cancer cells. Oncology Reports, 33(4): 1621-1629.
3. Dan S, Kang B, Duan X, Wang YJ. (2015) A cell-free system toward deciphering the post-translational modification barcodes of Oct4 in different cellular contexts. Biochemical and Biophysical Research Communications, 456(3):714-720.
4. Lin Y, Yang Y, Li W, Chen Q, Li J, Pan X, Zhou L, Liu C, Chen C, He J, Cao H, Yao H, Zheng L, Xu X, Xia Z, Ren J, Xiao L, Li L, Shen B, Zhou H, Wang YJ. (2012) Reciprocal regulation of Akt and Oct4 promotes the self-renewal and survival of embryonal carcinoma cells. Molecular Cell, 48(4):627-640.
5. Wang YJ, Li WH, Wang J, Xu K, Dong P, Luo X, Yin HL (2004) Critical role of PIP5KIg87 in InsP3-mediated Ca2+ signaling. Journal of Cell Biology, 167(6): 1005-1010.
6. Wang YJ, Wang J, Sun HQ, Martinez M, Sun YX, Macia E, Kirchhausen T, Albanesi JP, Roth MG, Yin HL (2003) Phosphatidylinositol 4 phosphate regulates targeting of clathrin adaptor AP-1 complexes to the Golgi. Cell, 114(3): 299-310.
7. Padron D, Wang YJ, Yamamoto M, Yin H, Roth MG (2003) Phosphatidylinositol phosphate 5-kinase Ib recruits AP-2 to the plasma membrane and regulates rates of constitutive endocytosis. Journal of Cell Biology, 162(4): 693-701.
8. Wang YJ, Gregory RB, Barritt GJ (2002) Maintenance of the filamentous actin cytoskeleton is necessary for the activation of store-operated Ca2+ channels, but not other types of plasma-membrane Ca2+ channels, in rat hepatocytes. Biochemical Journal, 363(Pt1): 117-126.
9. Wang J, Zhou L, Li Z, Zhang T, Liu W, Liu Z, Yuan YC, Su F, Xu L, Wang Y, Zhou X, Xu H, Hua Y, Wang YJ, Zheng L, Teng YE, Shen B. (2015) YY1 suppresses FEN1 over-expression and drug resistance in breast cancer. BMC Cancer, 15:50.
10. Su T, Dan S, Wang Y. (2014) Akt–Oct4 regulatory circuit in pluripotent stem cells. Chinese Science Bulletin, 59(10):936-943.
11. Cao H, Ma J, Yang J, Su X, Chen D, Yu J, Pan Q, Shao L, Zhou P, Li J, Wang Y, Li L. (2014) A metabonomics study of Chinese miniature pigs with acute liver failure treated with transplantation of placental mesenchymal stem cells. Metabolomics, 10(4):651-662.
12. Wang A, Chen F, Wang Y, Shen M, Xu Y, Hu J, Wang S, Geng F, Wang C, Ran X, Su Y, Cheng T, Wang J. (2013) Enhancement of Antiviral Activity of Human Alpha-defensin 5 against Herpes Simplex Virus Type 2 by Arginine Mutagenesis at Adaptive Evolution Sites. Journal of Virology, 87(5):2835-2845.
13. Yang Y, Li J, Pan X, Zhou P, Yu X, Cao H, Wang Y, Li L. (2013) Co-culture with mesenchymal stem cells enhances metabolic functions of liver cells in bioartificial liver system. Biotechnology & Bioengineering, 110(3):958-968.
14. Yang H, Liu C, Jamsen J, Wu Z, Wang Y, Chen J, Zheng L, Shen B. (2012) The DNase domain-containing protein TATDN1 plays an important role in chromosomal segregation and cell cycle progression during zebrafish eye development. Cell Cycle, 11(24):4626-4632.
15. Draz MS, Fang BA, Li L, Chen Z, Wang Y, Xu Y, Yang J, Killeen K, Chen FF. (2012) Hybrid nanocluster plasmonic resonator for immunological detection of hepatitis B virus. ACS Nano, 6(9):7634-7643.
16. Guo Z, Kanjanapangka J, Liu N, Liu S, Liu C, Wu Z, Wang Y, Loh T, Kowolik C, Jamsen J, Zhou M, Truong K, Chen Y, Zheng L, Shen B. (2012) Sequential posttranslational modifications program FEN1 degradation during cell-cycle progression. Molecular Cell, 47(3):444-456.
17. Yu J, Cao H, Yang J, Pan Q, Ma J, Li J, Li Y, Li J, Wang Y, Li L. (2012) In vivo hepatic differentiation of mesenchymal stem cells from human umbilical cord blood after transplantation into mice with liver injury. Biochemical and Biophysical Research Communications, 422(4):539-545.
18. Cao H, Yang J, Yu J, Pan Q, Li J, Zhou P, Li Y, Li J, Wang Y, Li L. (2012) Therapeutic potential of transplanted placental mesenchymal stem cells in treating Chinese miniature pigs with acute liver failure. BMC Medicine, 10:56.
19. Lu M, Felock PJ, Munshi V, Hrin RC, Wang YJ, Yan Y, Munshi S, McGaughey GB, Gomez R, Anthony NJ, Williams TM, Grobler JA, Hazuda DJ, McKenna PM, Miller MD, Lai MT. (2012) Antiviral activity and in vitro mutation development pathways of MK-6186, a novel nonnucleoside reverse transcriptase inhibitor. Antimicrobial Agents and Chemotherapy, 56(6):3324-3335.
20. Caulfield MJ, Dudkin VY, Ottinger EA, Getty KL, Zuck PD, Kaufhold RM, Hepler RW, McGaughey GB, Citron M, Hrin RC, Wang YJ, Miller MD, Joyce JG. （2010）Small molecule mimetics of an HIV-1 gp41 fusion intermediate as vaccine leads. Journal of Biological Chemistry, 285(52):40604-40611.
21. Lai MT, Lu M, Felock PJ, Hrin RC,1 Wang YJ, Yan Y, Munshi S, McGaughey GB, Tynebor RM, Tucker TJ, Williams TM, Grobler JA, Hazuda DJ, McKenna PM, Miller MD. (2010) Distinct mutation pathways of non-subtype B HIV-1 during in vitro resistance selection with non-nucleoside reverse transcriptase inhibitors. Antimicrobial Agents and Chemotherapy, 54(11):4812-4824.
22. Wang YJ, McKenna PM, Hrin R, Felock P, Lu M, Jones KG, Coburn CA, Grobler JA, Hazuda DJ, Miller MD, Lai MT. (2010) Assessment of the susceptibility of mutant HIV-1 to antiviral agents. Journal of Virological Methods, 165(2):230-237.
23. Cao H, Yu J, Xu W, Jia X, Yang J, Pan Q, Zhang Q, Sheng G, Li J, Pan X, Wang Y, Li L. (2009) Proteomic analysis of regenerating mouse liver following 50% partial hepatectomy. Proteome Science, 7:48.
24. Montgomery DL, Wang YJ, Hrin R, Luftig M, Su B, Miller MD, Wang F, Haytko P, Huang L, Vitelli S, Condra J, Liu X, Hampton R, Carfi A, Pessi A, Bianchi E, Joyce J, Lloyd C, Geleziunas R, Bramhill D, King VM, Finnefrock A, Strohl W, An Z (2009) Affinity maturation and characterization of a human monoclonal antibody against HIV-1 gp41. MAbs, 1(5):462-474.
25. Ingallinella P, Bianchi E, Ladwa NA, Wang YJ, Hrin R, Bonelli F, Ketas TJ, Moore JP, Miller MD, Pessi A (2009) Addition of a cholesterol group to an HIV-1 peptide fusion inhibitor dramatically increases its antiviral potency. Proc Natl Acad Sci USA, 106(14): 5801-5806.
26. Hrin R, Montgomery DL, Wang F, Condra JH, An Z, Strohl WR, Bianchi E, Pessi A, Joyce JG, Wang YJ (2008) In vitro synergy between peptides or neutralizing antibodies targeting the N- and C-terminal heptad repeats of HIV-1 gp41. AIDS Research and Human Retroviruses, 24(12): 1537-1544.
27. Su B, Hrin R, Harvey BR, Wang YJ, Ernst RE, Hampton RA, Miller MD, Strohl WR, An Z, Montgomery DL (2007) Automated high-throughput purification of antibody fragments to facilitate evaluation in functional and kinetic based assays. Journal of Immunological Methods, 322(1-2): 94-103.
28. Pizarro-Cerda J, Payrastre B, Wang YJ, Veiga E, Yin HL, Cossart P (2007) Type II phosphatidylinositol 4-kinases promote Listeria monocytogenes entry into target cells. Cellular Microbiology, 9(10): 2381-2390.
29. Yamamoto M, Chen MZ, Wang YJ, Sun HQ, Wei Y, Martinez M, Yin HL (2006) Hypertonic stress increases phosphatidylinositol 4,5-bisphosphate levels by activating PIP5KIb. Journal of Biological Chemistry, 281(43): 32630-32638.
30. Wang YJ, Gregory RB, Barritt GJ (2000) Regulation of F-actin and endoplasmic reticulum organisation by the trimeric G-protein Gi2 in rat hepatocytes: implication for the activation of store-operated Ca2+ inflow. Journal of Biological Chemistry, 275(29): 22229-22237.
31. Auld A, Chen J, Brereton HM, Wang YJ, Gregory RB, Barritt GJ (2000) Store-operated Ca2+ inflow in Reuber hepatoma cells is inhibited by voltage-operated Ca2+ channel antagonists and, in contrast to freshly isolated hepatocytes, does not require a pertussis toxin-sensitive trimeric GTP-binding protein. Biochimica et Biophysica Acta - Molecular Cell Research, 1497(1):11-26.